The photometric autotitration of free fatty acids.

A description is given of a new method for determining free fatty acids in blood plasma. The free fatty acids are extracted from the plasma, transferred to heptane and washed with H2SO4 (O.OlN). The earlier two-phase tritration procedure is now performed in a one-phase medium by means of an autoburette using tetrabutylammoniumhydrcxide. A curve, simultaneously recorded by means of a compensation recorder, allows for an accurate determination of the transition point of the titration, and accurate and reproducible measuring values can therefore be obtained. An unambiguous determination of small arterio-venous differences is likewise possible by this method.